摘要
目的 观察高糖状态下肾小球内皮细胞过氧化损伤与内皮细胞膜流动性改变的关系。方法 采用体外人肾小球内皮细胞培养 ,八木组织测量之TBA法测定脂质过氧化产物丙二醛 ,Prendergast法测定细胞膜流动性 ,Fura 2双波长荧光法检测细胞内钙离子含量的变化。结果 高糖对肾小球内皮细胞可致过氧化损伤 ,使细胞膜流动性明显增高 ,细胞内钙含量增加 ,随着培养时间的延长 ,糖浓度愈高 ,作用愈明显。结论 高糖致内皮细胞过氧化损伤影响细胞膜流动性改变 ,可能是糖尿病肾病发生的始动因素。
Objective To observe the retationship of lipoperoxidation induced injury of human glomerular endothelial cells (GEC) and the membrane fluidity of GEC in high concentration glucose. Methods The culture of human GEC was established, thiobarbituric acid fluoriphotometry was used to measured MDA, Prendergast's method was used to detect the membrane fluidity of GEC, and the [Ca 2+ ]i was determined by Fura 2/AM. Results The membrane fluidity and [Ca 2+ ]i of GEC were increased significantly in high concentration glucose induced injury of GEC by lipid peroxidation, and the higher the concentration of glucose, the more significant the effect as the cultured time went on. Conclusion The increased membrane fluidity caused by lipid peroxidation in high concentration glucose may be one of the initiating factors for diabetic nephropathogenesis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第6期723-725,共3页
Journal of Third Military Medical University
