摘要
目的 :建立反相高效液相色谱法测定补肾宁胶囊中淫羊藿苷的含量。方法 :采用水提取 聚酰胺小柱纯化去杂方法制备供试品溶液 ,以C18柱作为色谱柱 ,乙腈 水 (2 3∶77)为流动相 ,检测波长为 2 70nm ,按外标法进行检测。结果 :淫羊藿苷进样量在 0 .2 1- 1.91μg范围内线性关系良好 ,r =0 .9999;样品平均加样回收率为 99.2 1% (n =5 ) ,RSD值为 1.79% ;重复性试验RSD值为 2 .18% (n =5 )。结论 :方法简便 ,准确 ,重复性好 ,可作为补肾宁胶囊的含量测定方法。
Objective: To establish a RP-HPLC method for the determination of icariin in Bushenning Capsules. Methods: The sample was prepared by water extraction-purfication through polyamide column. The determination was carried out on C 18 ODS column with mobile phase of acetonirile-water (23∶77), and detection wavelength at 270nm according to the external standard method.Results: The icariin sample size showed a good linear relationship at the range of 0.12-1.91ug and correlation coefficient was 0.9999. The average recovery of the added sample was 99.21%(n=5) and RSD was 1.79%. The RSD in a duplicate test was 2.18%.Conclusion: The method is simple, accurate, reproducibe and can be used for content determination of icariin of icariin of Bushenning Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2001年第5期331-333,共3页
Chinese Traditional Patent Medicine
