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低密度脂蛋白胆固醇直接测定法的评价 被引量:3

Evaluation of direct method for measuring low-density lipoprotein cholesterol
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摘要 目的 评价基于选择性水解原理的两种低密度脂蛋白胆固醇 (LDL C)的直接测定法 (Ⅰ法和Ⅱ法 )的精密度、准确度和特异性。方法 将这两种方法与聚乙烯硫酸沉淀法 (PVS法 )作了比较 ,并以超速离心分离的HDL和LDL组分分析了对LDL C测定的特异性与准确性。结果 高、低两种LDL C浓度混合血清所测定的结果表明这两种方法均有良好的精密度 ,总CV值直接测定Ⅰ法 3 96 %~ 4 42 % ,直接测定Ⅱ法 0 78%~ 3 91% ,都可达到临床满意的程度。 48份临床标本测定结果 ( x±s)分别为 3 6 8± 1 2 3mmol/L(PVS法 ) ,3 2 5± 1 11mmol/L(直接测定Ⅰ法 )和 3 37± 1 2 1mmol/L(直接测定Ⅱ法 ) ,直接测定法与PVS法测定结果的差异无统计学意义。PVS法 (X)与直接测定Ⅰ法 (Y)的回归方程为Y =0 .848X +0 .12 1(r=0 .942 7) ,与直接测定Ⅱ法 (Y)的回归方程为Y =0 .914X +0 .0 0 7(r=0 .935 3) ,两种直接测定法与PVS法有良好的相关。对超离心HDL和LDL组分测定的结果表明两种直接测定法对LDL C有较好的特异性。稀释试验的结果表明两种直接测定法均有良好的线性 ,线性范围至少可达 9 2 8mmol/L。结论 以选择性水解法直接测定LDL C具有较好的精密度 ,对LDL有很好的特异性 ,适应自动分析 ,可以真实反映LDL C的水平 ,值得在临床推? Objective\ To evaluate the precise,accurate and specific of two direct methods for measuring low density lipoprotein cholesterol(LDL C)based on the principle of selective hydrolysys. Methods \ Both DAIICHI and Randox reagents were compared with PVS method and the ultracentrifugally separated HDL and LDL fractions were used.Results\ Both methods all had good precise,the total CV was 3.96%~4 42%(Daiichi)and 0.78%~3 19%(Randox),repectively.The average concentrations of 48 serum samples were 3.68±1.23 mmol/L(PVS method),3.25±1.11 mmol/L(DAIICHI method)and 3.37±1.21 mmol/L(Randox method),respectively.There was no statistics difference between the results from PVS method and other two direct methods.Furthermore it indicated that the results determined by both direct methods had good relationship with that by PVS method.It also indicated that both direct methods had good specific to LDL C.The dilute test demonstrated that there were good linearity between both direct methods of LDL C and the linearity range was 9.28 mmol/L at least.Conclusion\ The direct methods for determining LDL C based on the principle of selective hydrolysis possessed good precise and accurate and specific to LDL C,and was meet with clinical application.
出处 《临床检验杂志》 CAS CSCD 北大核心 2001年第3期152-154,共3页 Chinese Journal of Clinical Laboratory Science
关键词 低密度脂蛋白胆固醇 直接测定法 评价 LDL-C Low density lipoprotein cholesterol Direct measurement method Evaluation
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