摘要
Goldfish sperms were mixed with eggs for fertilization after incubation with antifreeze protein gene(AFP)from ocean pout for 30 min.A number of embryos and 145 adult goldfish were obtained.DNA from adult goldfish and embryos was extracted separately.Results of the amplification by PCRand Southern blot molecular hybridization indicate the integration of exogenous antifreeze gene intothe genome of a part of the recipient goldfish.Of the 45 samples detected by PCR,twelve showedpositive reaction with distinct hybridization band.The positive rate was 26%.
Goldfish sperms were mixed with eggs for fertilization after incubation with antifreeze protein gene (AFP)from ocean pout for 30 min.A number of embryos and 145 adult goldfish were obtained. DNA from adult goldfish and embryos was extracted separately.Results of the amplification by PCR and Southern blot molecular hybridization indicate the integration of exogenous antifreeze gene into the genome of a part of the recipient goldfish.Of the 45 samples detected by PCR,twelve showed positive reaction with distinct hybridization band.The positive rate was 26%.
