摘要
用非放射性的Digoxigenin标记的Ⅰ型马立克病病毒特异性DNA探针,杂交反应可有效地检出通过Southern blot及Dot blot固定到硝酸纤维膜上的同型病毒的DNA,其特性及敏感性均不低于放射性^(32)p标记的相同DNA探针。
With nonradioactive digoxigenin-labeled serotype I-specific MDV DNA fragment as probe, the hybridization could effectively detect serotype Ⅰ MDV DNA fixed on nitrocellulose filters by Southern blot or dot blot. Its specificity and sensitivity was as good as that with ^(32)P-labeled probe.
出处
《江苏农学院学报》
CSCD
1991年第1期1-6,共6页
Jiangsu Agricultural Research
关键词
DNA探针
病毒
马立克氏病
检出
Digoxigenin-labeling
DNA-probe
Marek's disease virus
