摘要
以奉化‘玉露’桃叶片、幼茎、下胚轴、子房、胚珠、胚乳及花后 4 5～ 5 0d和75d的未成熟胚、完全成熟的种胚为外植体 ,进行离体培养再分化试验。结果表明只有花后4 5～ 5 0d和 75d的未成熟胚产生白色致密节球状的愈伤组织 ,并可诱导出不定芽 ,不定芽分化率分别为 73.8%和 15 .5 % ;6 BA诱导愈伤组织不定芽的效果优于KT。不定梢转移生根成苗获得了完整的再生植株。
Various explants of‘Yulu’peach including leave,young shoot,hypocotyl,ovule,ovary,endosperm,immature embryo collected approximately 45-50 days or 75 days after full bloom,and mature embryo were cultured in vitro.As a result,only the white nodular callus derived from immature embryos collected 45-50 days and 75 days after bloom succeeded in producing adventitious buds,and the regeneration rates of white callus were 73.8% and 15.5% respectively.6 BA had stronger effect than KT in inducing adventitious bud formation.Adventitious shoots were transferred and cultured to plantlets.Effect of different hormone,carbon source,medium and transfer time on redifferentiation of adventitious buds from callus derived from immature embryos have been discussed.
出处
《园艺学报》
CAS
CSCD
北大核心
2001年第4期342-344,共3页
Acta Horticulturae Sinica
基金
国家自然科学基金资助项目 (39870 5 2 )
浙江省自然科学基金资助项目 (396 2 78)
农业部"九五"重点资助项目 (95农 17 0 1 0 4 0 3)
