摘要
采用聚丙烯酰胺垂直电泳法对120尾青海湖裸鲤进行了血清淀粉酶（AMY）电泳观察。结果表明：青海湖裸鲤血清淀粉酶有AMY1、AMY2和AMY3三种。AMY2同工酶在个体间存在多态性，其酶型分别为AMY2AA型(5．8％)、AMY2BB型(10．8％)、AMY2AB型(13．3％)和AMY2O型(70．0％)。AMY1和AMY3显现出单态。用同样的方法研究了鲤鱼和草鱼血清淀粉酶，结果其多态性谱带与青海湖裸鲤非常相似。认为，血清淀粉酶（AMY）在不同亚科鱼类间差异可能不显著。
s： 120 Qinghai－lake naked carps （Gymnocypris przewalskii Qinghai Lake Naked Craps）were used to investigate polymorphism of the blood serum amylase by means of polyacrylamide gel electrophoresis．The results were as follows： There were three isozymes including AMY1， AMY2， AMY3 in Qinghai－lake naked carps． There were four phenotypes including AMY2AA(5．8％)，AMY2BB(10．8％)， AMY2AB(13．3％) and AMY2O(70．0％) on the locus of AMY2， AMY1 and AMY3 were not found polymorphis existed．The analysis showed that the blood serum amylase could perhaps be species characteristics and not subfamily characteristics．
出处
《四川畜牧兽医》
2001年第9期15-15,17,共2页
Sichuan Animal & Veterinary Sciences
基金
国家自然科学基金(39860060)
教育部"高等学校骨干教师资助计划"项目资助。
关键词
青海湖裸鲤
血清淀粉酶
同工酶
多态性
： Qinghai－lake naked carps； blood serum amylase； isozyme； pol
