摘要
目的:了解胃癌组织HprueA基因表达状况,探讨HpureA基因与胃癌的关系。方法: 采用Warthin—Starry银染、原位PCR方法检测慢性浅表性胃炎(CSG)35例、慢性萎缩性胃炎(CAG)40例、胃癌(CG)35例胃粘膜Hp及ureA基因感染状况。观察非癌患者胃粘膜炎症反应程度。结果: 慢性浅表性胃萎缩性胃炎、胃癌组织、癌旁胃粘膜组织Hp检出率分别为48.57％、62.5％、0、54.28％;ureA基因检出率分别为51.42％、75％、37.14％、82.86％,萎缩性胃炎、癌旁组ureA基因检出率显著高于浅表性胃炎组(P<0.05,P<0.01)。非癌患者胃粘膜单个核细胞、分叶核细胞浸润程度ureA组、Hp+级较HP组显著增多(P<0.01)。结论: 萎缩性胃炎、胃癌患者HpureA基因表达显著高于慢性浅表性胃炎组。Hp感染引起更严重的炎症反应。说明Hp感染与萎缩性胃炎、胃癌关系密切.在胃癌的发生中起着重要作用。
To examine Helicobacter pylori (Hp) ureA gene expression in gastric cancer tissues so as to study the relationship between HpureA gene and gastric carcinoma. Method: Warthin - Starry silver staining was used to detect Hp, and in situ poly-merase chain reaction to detect HpureA gene in gastric mucosa from 35 cases of chronic superficial gastritis, 40 cases of chronic atrophic gastritis, 35 cases of gastric carcinoma. Result: The rates of Hp infection were 48.57% in chronic superficial gastritis, 62.5% in chronic atrophic gastritis, 0 in carcinoma tissue, 54.28% in gastric mucosa beside carcinoma tissue. The positive rates of HpureA gene were 51.42% in chronic superficial gastritis, 75% in chronic atrophic gastritis, 37.14% in carcinoma tissue, 82.86%in gastric mucosa beside carcinoma tissue. In regard to HpureA gene expression, a significant difference were observed between chronic superficial gastritis, and chronic atrophic gastritis (p < 0.05) and gastric cancer (p < 0.01). In those cases without gastric carcinoma, higher scores of mononuclear and polymorphonuclear cells in gastric mucosa were observed in ureA group than in ureA group (p < 0.01), and in Hp + group than in Hp group (p < 0.05) . Conclusion: A significant higher espression of HpureA gene was observed in chronic atrophic gastritis, in gastric cancer than in chronic superficial gastritis. Hp infection can cause a significant inflammatory response.
出处
《西南国防医药》
CAS
2001年第3期160-163,共4页
Medical Journal of National Defending Forces in Southwest China
