摘要
目的 本研究旨在评价血清脂肪酸结合蛋白 (FABP)定量检测对急性心肌梗死 (AMI)患者早期诊断价值。方法 采用酶联免疫吸附试验一步夹心法对 2 0名健康体检者、2 6例AMI患者进行血清FABP检测 ,同时测定心肌肌钙蛋白T(cTnT)、肌酸激酶同工酶MB(CK MB) ,并对其中 9例AMI胸痛发作后 6h内入院患者血清进行动态分析。结果 2 0名健康体检者FABP、cTnT、CK MB血清浓度分别为 (1 8± 0 9) μg/L、(0 16± 0 10 ) μg/L、(15 2± 3 8)U/L ,2 6例AMI患者血清浓度分别为 (38 6±2 1 8) μg/L、(2 5± 2 4) μg/L和 (90 5± 83 5 )U/L ,阳性率为 10 0 %、84 5 %和 80 8%。 9例动态观察的患者中 ,有 4例 1h内入院时血清中FABP浓度开始升高 [(14 1± 4 1) μg/L],9例患者血清升高达峰值时间为 (7 0± 3 2 )h ,比cTnT、CK MB早 (P <0 0 0 1) ,2 4～ 48h恢复正常。
Objective To evaluate the early diagnostic value of serum fatty acid binding protein (FABP) in acute myocardial infarction (AMI) Methods Serum FABP was measured in 20 healthy subjects and 26 AMI patients by ELISA one step sandwich assay Cardiac troponin T (cTnT) and creatine kinase MB (CK MB) were measured at the same time Nine out of the 26 patients who were taken in after onset of chest pain within 6 hours were regularly detected Results Serum concentrations of FABP, cTnT, CK MB were (1 8±0 9) μg/L, (0 16± 0 10) μg/L and (15 2±3 8) U/L, respectively in the 20 healthy subjects They were (38 6±21 8) μg/L, (2 5±2 4) μg/L and (90 5±83 5) U/L, respectively in the 26 AMI patients whose positive rates were 100%, 84 5% and 80 8% The concentrations of serum FABP in 4 patients were elevated [(14 1±4 1) μg/L] whthin 1 hour The peak of concentrations of serum FABP appeared within (7 0±3 2) h in 9 patients, earlier than that of cTnT, CK MB( P <0 001), which reached normal within 24～48 h Conclusion FABP can become a good serum marker for early diagnosis of AMI
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2001年第4期220-222,共3页
Chinese Journal of Laboratory Medicine
基金
江苏省社会发展指导性计划项目 (BS2 0 0 0 3 5 9)
