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儿茶素对溶血卵磷脂胆碱所致细胞损伤的保护作用 被引量:2

Protective Effects of Catechin on Vascular Endothelial Cells Against Lysophosphatidylcholine Damages
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摘要 作者以体外培养的小牛主动脉内皮细胞为材料 ,研究了溶血卵磷脂胆碱 (LPC)和氧自由基(OFR)对血管内皮细胞 (VEC)的损伤 ,及儿茶素对VEC的保护。结果显示 :当VEC与LPC(5ug/ml)或黄嘌呤 /黄嘌呤氧化酶 (X/XO) (10umol/L + 2 0 0umol/L)共孵育 2 4小时时 ,表现为细胞内乳酸脱氢酶 (LDH)泄漏量增多 ,细胞内过氧化脂质丙二醛 (MDA)含量升高 ,细胞生长减缓 ,存活率下降 ;当加入不同浓度的儿茶素后则可明显抑制LDH的泄漏量 ,降低MDA含量 ,细胞生长正常 ,存活率提高。表明LPC与X/XO对VEC有损伤作用 ,而儿茶素则能通过抗氧化途径抵抗LPC与X/XO致VEC的损伤。 Effects of catechin on cultured bovine endothelial cells damaged by lysophosphatidylcholine(LPC) or oxygen free radicals(OFR) were investigated. When vascular endothelial cells(VEC) was incubated with LPC(5ug/ml) or xanthine/xanthine oxidase(X/XO) (10umol/L+200umol/L)for 24 hours, the contents of lactate dehydrogenase(LDH) and molondialdehyde(MDA) were markedly increased, the VEC grew slowly. Treatment with different concentrations of catechin caused a reduction in LDH and MDA contents, the VEC grew normally. There results suggest that catechin afforded protection against VEC damages induced by LPC or X/XO and that the protective effect of catechin may be due to anti-lipid peroxidation via free radicaed scavenging activity.
出处 《华东师范大学学报(自然科学版)》 CAS CSCD 北大核心 2001年第3期97-101,共5页 Journal of East China Normal University(Natural Science)
基金 上海现代生物与医药产业办公室资助项目
关键词 儿茶素 血管内皮细胞 溶血卵磷脂胆碱 细胞损 保护效应 药理作用 抗氧化剂 catechin vascular endothelial cells lysophosphatidylcholine
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