摘要
建立了一种快速、灵敏、连续测定血清中铜和铁含量的分光光度法。铜、铁与 5-Br -PADAP及TritonX - 10 0在pH 4 0缓冲溶液中生成红色配合物 ,测定吸光度 (A1) ,加入掩蔽剂后再测定吸光度 (A2 ) ,A2 值与铁离子浓度成正比 ,吸光度下降值 (△A =A2 -A1)与铜离子浓度正比。铜、铁配合物表观摩尔吸收系数分别为 6 4 4× 10 4 、 6 53× 10 4 L·mol- 1·cm - 1,铜、铁含量分别在 0～ 80、 0～ 70 μmo1·L- 1范围内性线良好 ,变异系数分别为 2 7%、 2 2 % ,平均回收率为10 0 8%、 10 0 7% ;铜、铁含量测定分别与双环己酮草酰二腙法、亚铁嗪法相比较 ,无显著性差异 (P >0 0 5)。
To create a swift and sensitive spectrophotometry for the continuous determination of copper and iron in serum, copper and iron reacted with 5-Br-PADAP and TritonX-100 in the HAc-NaAc buffer medium of pH 4 0, and formed a red complex, the system’s absorbance ( A 1)was determined and added the masking reagent into it, the absorbance( A 2)was determined again The value of ( A 2) was directly proportional to the concentration of iron The absorbent declining volume (△ A = A 2 - A 1)was directly proportional to the concentration of copper With this method, the complex of copper and iron’s apparent molar absorptiviy is 6 44×10 4, 6 53×10 4 L·mol -1 ·cm -1 , CV was 2 2%, 2 7%, the average rate of recovey was 100 8%, 100 7% As compared with biscyclohexanone oxalydihydrazone spectral method and ferrospectral method separately, there was no significant difference among the methods( P > 0 05)
出处
《广东微量元素科学》
CAS
2001年第1期50-53,共4页
Trace Elements Science
