摘要
本文以随机引物成对组合的方式 ,对和缓艾美耳球虫 (Eimeria .mitis)、巨型艾美耳球虫 (E .maxima)和柔嫩艾美耳球虫 (E .tenella ) 3个种各 2个单卵囊感染纯株、E .tenella 6个抗药性表型不同的单卵囊感染纯株进行了RAPD分析 ,结果发现 ,RAPD技术能非常有效地用于上述球虫种、株间的鉴别。
A genetic polymorphism study was performed in coccidia from the domestic fowl by random amplified polymorphic; DNA(RAPD)using pairwise combinations of primers.Two strains each of Eimeria mitis,E.maxima and six strains of E.tenella that differ markedly in drug_resistance phenotype were used.The results suggest that the RAPD assay could be an extremely useful tool for the study of differentiation of these coccidia species and strains.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2001年第5期359-363,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然科学基金资助项目 (30 0 0 0 12 4)
关键词
鸡球虫
RAPD
抗药性
种株
多态性
Coccidia
Random amplified polymorphic
DNA(RAPD)
Drug_resistance
Species
Strain
