摘要
目的 6 β-乙酰氧基去甲托烷 (6 β -AN)是新合成的外消旋莨菪烷衍生物 ,实验表明为M受体激动剂。本课题从离体水平 ,对其化学拆分后的左旋体 (1- 6 β -AN)和右旋体 (d - 6 β -AN)对家兔虹膜上M胆碱受体的作用进行研究 ,来阐明其作用机制。方法 制备离体虹膜标本 ,用累计浓度法制作量效关系曲线。结果 1- 6 β -AN对离体虹膜括约肌呈现明显的浓度依赖性收缩 ,阿托品可使其收缩曲线平行右移 ;d - 6 β -AN仅在较大剂量时对虹膜有微弱的收缩作用 ,对 1- 6 β -AN也不表现为拮抗作用。结论 1- 6 β -AN致虹膜收缩主要是激动虹膜上M受体所致 ;d - 6 β
Objective 6β-Acetoxy nortropane (6β-AN)is a newly synthesized derivative of nortropane. Animal experiments have shown that it is an agonist of M-cholinergic receptor. Effects of L-6β-AN and d-6β-AN on the M-cholinergic receptor on irises of rabbits were studied in vitro, and illuminated the mechanism of its action. Methods To prepare the isolated iris specimen and draw dose-effect relationship curve with a concentration-accumulated manner. Results On isolated irises of rabbits, L-6β-AN caused a concentration-dependent contraction which was completely antagonized by M-receptor blocker atropine. It shifted the dose-response curve of L-6β-AN to the right, d-6β-AN can caused the irises feeble contraction only in a large dosage and d-6β-AN did not antagonize the effects L-6β-AN. Conclusions The isolated iris contraction effect induced by L-6β-AN mainly come from activation of M-receptor on the irises.D-6β-AN showed no apparent contraction effect on the irises.
出处
《齐齐哈尔医学院学报》
2001年第4期362-363,共2页
Journal of Qiqihar Medical University
