摘要
利用分子活化分析技术初步研究了天然植物铁芒萁叶中稀土元素与蛋白、多糖和DNA的结合状态。铁芒萁叶经过提取和生化分离后 ,得到了稀土结合蛋白、稀土结合多糖和稀土结合DNA。用中子活化法测定 8个稀土元素 (La、Ce、Nd、Sm、Eu、Tb、Yb和Lu)的含量。研究结果表明 ,铁芒萁叶子中的稀土元素与蛋白质、多糖及DNA等生物大分子的结合是紧密的。经分子量测定 ,与稀土元素结合的两种蛋白的分子量分别为 8.0× 10 5u和 <12 4 0 0u ;与稀土结合的多糖为小分子量多糖 ,分子量为 10 0 0 0— 2 0 0 0 0u。生化分离得到的REE -DNA ,经琼脂糖凝胶电泳分离 ,得到一条很清晰的DNA谱带 ,分子量约为 2
The binding of rare earth elements with proteins, polysaccharides (PSs), nucleic acid (DNA) in natural plant Dicranopteris Dichotoma (DD) was studied by molecular activation analysis (MAA). The REE bound proteins (RBP), polysaccharides (PSs) and DNA (REE-DNA) were obtained using biochemical separation techniques from the leaves of DD. Meanwhile, the contents of eight rare earth elements (La, Ce, Nd, Sm, Eu, Tb, Yb and Lu) were also measured by instrumental neutron activation analysis (INAA). The results showed that the REEs were firmly bound with the proteins, PSs and DNA in the leaves of DD. The molecular weights of RBP-Ⅰ and RBP-Ⅱ were 8.0×10 5u and <12400u, respectively. Most of the PSs bound with REEs were small molecular weight PSs (10000 to 20000u). The molecular weight of REE-DNA band was about 22kb in agarose gel electrophoresis.
出处
《核技术》
CAS
CSCD
北大核心
2001年第9期721-726,共6页
Nuclear Techniques
基金
国家自然科学基金 (19935 0 2 0 )资助
