摘要
目的 研究大枣中性多糖（JDP-N）对小鼠腹腔巨噬细胞（Mφ）分泌肿瘤坏死因子（TNF）及其mRNA表达水平的影响。方法 MTT法测定细胞增殖，半定量RT-PCR测定TNF-α mRNA的表达。结果 JDP-N能诱导Mφ分泌TNF，诱生TNF达峰时间约为6 h，与脂多糖相比，时效关系相仿；蛋白激酶C激活在JDP-N诱导Mφ分泌TNF过程中具有关键性作用；JDP-N能促进MφTNF-α mRNA的表达。结论 JDP-N增强小鼠免疫功能的机制之一是促进Mφ分泌TNF。
Objective To understand whether jujube date polysaccharide (JDP-N) affects tumor necrosis factor (TNF) secretion and its mRNA expression in mouse peritoneal macrophages Mφ． Methods Cell proliferation was determined by MTT method and TNF-α mRNA by semiquantified RT-PCR. Results JDP-N stimulated TNF production that reached the maximum at around 6 h, demonstrating similar effect to that of lipopolysaccharide on TNF production. Protein kinase C played a key role in mediating TNF production of Mf induced by JDP-N, in the process of which enhancement of TNF-a mRNA expression in Mφ was also observed. Conclusion Promotion of TNF production might be one of the mechanisms by which JDP-N enhances the immune function.
出处
《第一军医大学学报》
CSCD
北大核心
2001年第8期592-594,共3页
Journal of First Military Medical University
