摘要
目的 了解中药成分华蟾毒精 (cinobufagin,CBG)的体外抗癌活性及其主要作用机制 .方法 采用流式细胞术检测华蟾毒精对体外培养的人肝癌细胞株 SMMC- 772 1、人白血病细胞株 HL- 60的细胞毒作用 ;经透射电子显微镜观察CBG作用后培养的 SMMC- 772 1及 HL- 60细胞超微结构的改变 .结果 华蟾毒精有显著的抗癌活性 ;对 HL- 60细胞的IC5 0 为 1.2 5μg· L- 1 ,对 SMMC- 772 1细胞的 IC5 0 为 2 .72μg· L- 1 ,能非常显著地减少 HL- 60 ,SMMC- 772 1细胞 S期DNA含量及增值指数 ,诱导癌细胞凋亡 ;透射电镜观察显示 :华蟾毒精作用后 ,可见成片的细胞坏死 ,细胞凋亡 ,内质网肿胀、线粒体肿大呈空泡样 ,溶酶体增多等细胞结构改变 .结论 华蟾毒精有确切的抗癌作用 ,其作用产生的机制是多方面的 ,既能抑制癌细胞 DNA的生物合成 。
AIM To explore anticancer activity and mechanism of Cinobufagin (CBG) in vitro . METHODS Effect of CBG on the DNA content in S phase of cell cycle and the proliferative index of human hepatocarcionoma cell strain SMMC 7721 and human leukemia cline HL 60 cultuerd in vitro and the effect on inducing the cancer cell to apoptosis were checked up by flow cytometry the cytotoxic reaction of CBG on SMMC 7721 and HL 60 were investgated by the method of MTT. And the changes of the cellar ultrastructure induced by CBG were observed by the transmission electron microscopy. RESRLTS CBG had the remarking anticancer activity. The IC 50 of CBG on HL 60 was 1.25 μg·L -1 and the IC 50 on SMMC 7721 was 2.72 μg·L -1 . And the DNA content and the proliferative index of HL 60 SMMC 7721 were evidently reduced. The cancer cell was induced to apoptosis by CBG. The changes of organelle structure, a lot of necrosis and apoptosis of cells, swelling of mitochondrial and endoplasmic reticulum, and increasing of lysosme could be observed under the transmission electron microscopy. CONCLUSION CBG has an efficacious anticancer mechanism, which may be carried out by inhibiting the DNA biosynthesis of cancer cell and inducing the cell apoptosis.
出处
《第四军医大学学报》
北大核心
2001年第16期1504-1507,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (3 9470 85 3 )
关键词
抗癌作用
实验研究
华蟾蜍毒素
肝肿瘤
白血病
比色法
流式细胞术
显微镜检查
cinobufagin
liver neoplasms
leukemia
cells
colorimetry
nitroblue tetrazolium
flow cytometry
microscopy, electron, scanning transmission
