摘要
目的 探讨通过肝缺血 /再灌期 ( ischem ia and reperfusion I/ R)给予药物预处理动物模型来探索不同药物、不同给药途径防治肝脏 I/ R期损伤的有效方法。方法 60只 Wistar大鼠分成 5组 :A组 (正常对照组 )、B组 ( I/ R期无药物干预模型组 )、C( 10 % L- arg静注干预组 )、D组 ( 1% SF静注干预组 )、E组 ( 2 % SF实验前 3 d口服干预组 ) ,动脉夹阻断肝门 4 5 min后去除动脉夹再灌注 1h。于阻断肝门前、阻断后 4 5 m in、再灌注 1h测定肝脏微循环血流量 ,检测血清 AL T、AST值 ,同时取测定点处肝脏组织病理进行光镜、电镜观察分析。分别用统计学 t检验及单因素方差析的方法比较 5组间的差异和意义。结果 阻断肝门前 E组肝脏微循环血流量明显高于 A、B、C、D组 ( P<0 .0 1) ,阻断 4 5 m in时 B、C、D、E组微循环血流量均呈一致下降趋势 ,E组再灌注 1h后回升至 2 8.3 9± 0 .83 ( V) ,与 B组 2 4 .71± 0 .4 5 ( V)比较明显增高 ( P<0 .0 5 )。病理分析证实 I/ R期肝组织损伤主要发生在再灌注初期肝小叶的中央静脉区及肝腺泡 区 ,光镜半定量分析显示 E组肝细胞坏死数目明显减少 ( P<0 .0 5 )。结论 阻断肝门后肝脏微循环明显下降 ,再灌注早期可回升 ,但不能达到正常值。黄腐酸钠 ( SF)
Objective To try to find an effective way to ease the impairment of I/R in liver by medicine in advance.Methods Ischemia and reperfusion are produced by blocking the hepatic portal with microvascular clamp for 45min after abdominal cavity anesthesia,then reperfusion for 1 h was detected.Then,the level of ALT and AST in plasma by drawing blood from inferior vena cava after reperfusion for 1 h,meanwhile the hepatic pathological tissue was analyzed semiquantitatively .All experimental rats,which consist of 5 groups according to taking medicine:group A(normal control group),Group B(I/R model group),Group C(10%L-arg vein injection for three days),Group D(1% SF vein injection),Group E(2%SF continual taking orally).The data were dealt with t and square deviation tests to distinguich if there were significant difference among these 5 groups.Results It was showed that Group E can increase hepatic microcirculation not only during normal condition but also during reperfusion.Their microbloodflow were as follows respectively:40.75±1.05 vs. 33.04±1.53 V(P<0.01),28.39±0.83 vs. 24.71±0.45 V(P<0.05).The hepatic pathological study showed:the injuried tissue mainly amass in portal area and hepatic acinus Ⅲ,the amount of hepatocyte necrosis in Group E is much less than that in Group B,D(P<0.05),as confirmed by microscope observation.Conclusion After blocking hepatic portal,hepatic microcirculation decreases remarkably,then returns to rise during reperfusion,but can not restore to normal.Sodium Fulvate (SF) can increase hepatic microcirculation not only during normal condition but also during reperfusion and improve remarkably hepatic pathological impairment.It might be a good medicine for preventing impairment of hepatic ischemia and reperfusion.
出处
《肝胆外科杂志》
2001年第4期311-313,共3页
Journal of Hepatobiliary Surgery
关键词
肝脏微循环
肝缺血
再灌注
黄腐酸钠
Hepatomicrocirculation
Ischemia and Reperfusion
Sodium fulvate
