摘要
用枯草杆菌中性蛋白酶（ＡＳ１３９８酶）提取鱼蛋白酶水解物。酶水解条件由正交实验确定，即鱼匀浆用ＡＳ１３９８酶３５０ＩＵ／ｍｌ在４２℃下水解１２ｈ。水解液经活性炭吸附结合β－环糊精掩盖脱去苦味。生产性中试蛋白质回收率７８．８０％，喷雾干燥所得制品产率为１４．９１％。制品呈白色粉末，无苦味；蛋白质含量８８．２６％，必需氨基酸占总氨基酸４３．６１％，氨基酸间比例合理。鱼蛋白酶水解物为优质蛋白质制品。
A fish protein hydrolysate was priqared from Anchovy Engraulis japonicus by using a neutral proteinase from Bacillus subtilis (AS1398-ase). The optimal conditions of enzymatic protein hydrolysis were selected by applying the method of orthogonal experiments. The process was as follows:the homogenate of Anchovy was hydrolyzed with an amount of 350IU/ml of AS1398-ase at 42℃ for 12 hours. The hydrolysate was debittered by using active carbon and β-cyclidextrin consecutively. A small- scale industrial prouction was carride out and it was found that the fecovery rate of protein was up to 78. 80%,and the yield of the end product gained by spray-dry was 14. 91%. The product is a non-bitter white powder containing 88. 26% of protein. The essential amino acids amounted to 43. 61 %of the total aminoi acids in the product,and the ratio among the essential amino acids was appropriate. The product was high quality protein prodrct.
出处
《水产学杂志》
CAS
1996年第1期7-11,共5页
Chinese Journal of Fisheries
关键词
Ti鱼
蛋白酶水解物
酶解法
提取
蛋白质制品
Anchovy Engraulis japonicus, Enzymic protein hydrolysate, Enzymatic hydrolysis
