摘要
利用聚合酶链反应（ＰＣＲ）对１３５例多类临床标本（痰、胸水及尿等）进行了检测。临床疑诊为结核病者１０５例，其中１８例结核杆菌涂片阳性，ＰＣＲ检测均为阳性；８７例结核杆菌涂片阴性的标本，５７例ＰＣＲ检测阳性，总阳性率为７１．４％。对照的３０例非结核病的标本则无阳性。本研究结果表明，ＰＣＲ在敏感性，特异性方面均优于传统的结核病诊断方法。它简捷、易行、直观，尤其适用于结核病的早期、快速诊断。
PCR technique was used to detect the repetitive DNA sequences of Mycobacterium tu-berculosis in various clinical specimens of patients with tuberculosis. The amplification proto-col gave positve results in all of eighteen cases positive by smar and in 57 of 87 cases negativeby smear. The total positive rate detected by PCR wsa 71.4%and o in 30 controls.The re-sults shoued PCR was more sensitiv and specific than conventional methods in dete- cting My-cobacterium tuberculosis.It is simple,easy to perform and directly perceived by vision,espe-cially suitable in early and rapid diagnosis of tuberculosis.
出处
《江西医学院学报》
CAS
1994年第4期17-20,F003,共5页
Acta Academiae Medicinae Jiangxi
基金
江西省卫生厅希望基金
关键词
聚合酶链反应
结核病
实验室诊断
PCR法
polymerase chain reaction￣*/method
tuberculosis/diagnosisdiagnosis,laboratory* complement words
