摘要
目的 研究大鼠肝再生增强因子（ａｕｇｍｅｎｔｅｒ ｏｆ ｌｉｖｅｒ ｒｅｇｅｎｅｒａｔｉｏｎ，ＡＬＲ）在大鼠基因组中的存在方式。方法 以大鼠基因组ＤＮＡ为模板，根据ＡＬＲ ｃＤＮＡ序列设计引物，用多聚酶链反应（ＰＣＲ）扩增产物，连入 ｐＧＥＭ Ｔｅａｓｙ Ｖｅｃｔｏｒ后测序。结果 ＰＣＲ法扩增出两条产物，其一经测序发现为ＡＬＲ的假基因，预测氨基酸序列与ＡＬＲ的同源性为８８．８％。结论 作为肝细胞再生过程中重要的生长刺激因子，大鼠ＡＬＲ存在假基因，提示可能存在ＡＬＲ多基因家族。为研究ＡＬＲ分子的进化规律提供了依据。
Objective: To investigate the status of the augmenter of liver regeneration (ALR) in rat's genome. Methods Polymerase chain reaction (PCR) was used to amplify the genomic DNA of rat, with a set of specific primers designed according to the cDNA sequence of ALR. The products were ligated into pGEM Teasy vector. Two positive clones were sequenced separately. Results Two products were amplified from the rat's genome by PCR. After sequencing, one pseudogene was identified. The homology of the amino acid sequence between the ALR and its pseudogene was 88.8%. Conclusions ALR pseudogene is found in rat's genome, implying that there is an ALR multigene family. This finding lays a foundation for further study of ALR molecular evolution mode.
出处
《中华肝脏病杂志》
CAS
CSCD
2001年第2期105-107,共3页
Chinese Journal of Hepatology
