摘要
目的 探讨不同G值重复暴露后大鼠脑神经元损伤及其区域分布特点 .方法 将 5 3只雄性SD大鼠随机分为对照组、+2Gz,+6Gz,+10Gz和 +10Gy暴露组 .分别在暴露后 6h ,1d ,3d处死取脑 ,做石蜡或电镜超薄切片 ,利用光镜和电镜 ,观察不同G值重复暴露后 ,脑神经元形态学和超微结构的变化 .结果 +2Gz重复暴露组无明显的脑神经元形态学变化 .+6Gz重复暴露后 6h可见脑神经元超微结构的轻度变化 ,2 4h恢复正常 .+10Gz和 +10Gy重复暴露后 ,分别在海马CA1区、下丘脑、梨状皮层和左颞叶皮层、左梨状皮层 ,可见一定数量的变性神经元及重度的超微结构损伤 .结论 高G值重复暴露可引起大鼠特定脑区的神经元损伤 。
AIM To investigate the area distribution peculiarity of rats brain injury after repeated +Gz exposures. METHODS 53 SD male rats were divided into control group, +2 Gz, +6 Gz, +10 Gz and +10 Gy exposure group. Rats brains were taken 6 h, 1 d or 3 d after +Gz exposure run for histopathologic and ultrastructure studies by light microscope and electron microscope. RESULTS There were not obvious changes of neuron morphology after repeated +2 Gz exposures. At 6 h after repeated +6 Gz exposures, there were gently ultrastructure changes in rats brain, and resumed at 24 h. We could find a lots of degeneration neurons with weightily ultrastructure injury at hippocampus CA1, hypothalamus, piriform cortex, and left temporal cortex and left piriform cortex after repeated +10 Gz or +10 Gy exposures. CONCLUSION There was neuron injury in special brain area after repeated high G exposure. It could be caused by mechanal force effect.
出处
《第四军医大学学报》
北大核心
2001年第15期1436-1439,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (3980 0 1 58)
关键词
加速度
组织病理
组织超微结构
脑
acceleration
histopathology
ultrastructure
brain
rats
