摘要
目的 研究胰腺癌组织中ARHI的mRNA水平与蛋白表达情况。方法 用单克隆抗体免疫组化及地高辛标记cDNA探针原位杂交的方法，对57例胰腺癌手术切除组织进行ARHI蛋白及mRNA检测，并以正常胰腺组织作对照。结果 ARHI在正常胰腺组织中的免疫组化及原位杂交阳性率分别为84．2%及82．5%；在胰腺癌中两者阳性率均为52．6%，阳性率显著下降（P＜0．01）；ARHI的mRNA与蛋白表达有较好的一致性；此外，在纤维母细胞、平滑肌细胞、血管壁及肠粘膜上皮均可见阳性着色；ARHI的阳性率与癌分化程度及临床分期无关。结论 ARHI在胰腺癌中呈下调性表达，可能是一个抑癌基因；ARHI广泛存在于腺上皮及纤维、平滑肌等细胞中。
Objective To investigate ARHI mRNA and protein expression in pancreatic cancers． Methods Fifty-seven paraffin-embedded resected cancer samples and patient-corresponding normal pancreatic tiss- ues were determined by using immunohistochemistry staining and in situ hybridization method． Results The positive rate of ARHI mRNA and protein expression in normal control pancreatic tissue was 84．2% and 82．5% respectively, and in cancer group was 52．6% both． The positive rate of mRNA and protein expression in cancer group was markedly decreased(P ＜ 0．01)． Yet the protein expression correlated with mRNA． Furthermore, positive staining of ARHI protein and mRNA can be observed in duodenal mucus epithelium, fibroblasts, liomyocytes, and wall of blood vessels． There was no correlation between positive rate and cancer differentiation, or clinical stages． Conclusions ARHI protein expres- sion is down-regulated in pancreatic cancer, possibly plays a role as tumor suppress gene, ARHI pro- tein expressed in multiple types of tissues including duodenal mucus epithelium, fibroblasts and so on．
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2001年第4期324-327,共4页
Acta Academiae Medicinae Sinicae
