摘要
用重组DNA技术构建了鸭乙型肝炎病毒(DHBV)的Pre S/S的表达质粒——D.F.W.29,表达的融合蛋白分子量为30kd。用SDS-PAGE分离细菌蛋白,经考马斯亮蓝染色、激光光密度扫描定量分析表明,DHBV的pre S/S表达的融合蛋白占细菌总蛋白的16字。将此融合蛋白免疫家兔,可获得相应抗体。免疫印迹法(Western blot)测抗体灵敏度,证明和该抗体呈现阳性反应的最低抗原量为3μg。用此抗体检测鸭血清中DHBsAg的初步结果表明,它与鸭血清DHBV DNA斑点杂交结果基本相符。因此,该抗体可以用作检测鸭乙型肝炎病毒携带状态并可用于研究鸭肝癌与肝炎的关系。
A DHBV preS/S expression plasmid(D F.W.29)was constructed with vector PEX34a. SDS-PAGE and laser scanning showed a unique band of fusion protein (MW 30000)accounting for 16% of total bacterial proteins.Antibody against the fusion protein was prepared by immunizing rabbits with the gel band and testified with Western blot analysis. The oc-currence of DHBsAg in duck serum samples was determined by Westera blot. Our data seem to be mostly consistent with those obtained by dot hybridization of DHBV-DNA. The antibody against the fusion protein therefore, can be usad to reveal DHBV carrier conditions and tin relationship between duck hepatitis B and liver cancer.
出处
《病毒学报》
CAS
CSCD
北大核心
1989年第3期211-216,共6页
Chinese Journal of Virology
