摘要
对栀子（Gardenia jasminoides)不同部位外植体进行无菌苗的诱导培养，诱导率表现为带腋芽的茎段＞顶芽，而叶片则不能被诱导发芽。在继代过程中培养基MS＋BA2＋NA0．5的继代效果好于培养基MS＋KT0．02＋NAA1．5。生根培养基1／2MS＋IBA0．5＋NAA0．1和1／2SH…IBA0．5＋NAA0．1均有良好的生根效果。移栽时，基质温度掌握在18－20℃能够保证试管苗有良好的长势。
The different parts of explant of Gardenia jasminoides were made induced culture. Inductive rate presents stem with auxiliary bud>terminal bud, but leaves can't be budded off. In the process of subculture, the subculture effect of culture medium MS+BA 2+NAA 0.5 is better than that of culture medium MS+KT 0.02 +NAA 0.5 . Culture medium of root initiation 12MS+IBA 0.5 +NAA 0.1 and 12SH+IBA 0.5 +NAA 0.1 have better effect. Tube seedlings have good growth under the condition of transplanting. The temperature of culture medium with 18～20?℃ during the process.
出处
《北华大学学报（自然科学版）》
CAS
2000年第6期533-535,共3页
Journal of Beihua University（Natural Science）
基金
吉林省林业厅项目!(吉林科字 980 17号 )
