摘要
草鱼出血病病毒感染鱼肾(Clk)单层细胞后,产生明显的细胞病变,其繁殖周期为2—3天。病毒增殖的上升期在感染后的12—48小时。28℃下,当感染复数(Multiplicityof Infection简称MOI)为0.05PFU/cell时,病毒滴价可达1.78×10^(11)PFU/ml,不同温度(25℃、28℃、30℃)下通气培养,测定病毒滴价略有差异。感染病毒的细胞培养液经ELISA检测和电子显微镜观察均为阳性结果,并且能感染健康草鱼。
Some biological properties of Grass Carp Hemorrhage Virus (GCHV)-873have been studied in this report. This virus could make grass carp kidney cells(CIK) to produce a unique cytopathic effect. At 28℃ its logarithmic growthphase occured 12 to 48 hr after infection. The virus titer reached 1.78×10^(11)PFU/mlwhen the MOI was approximately 0.05PFU/cell. There were slight differencesin virus titer under incubation of 25℃, 28℃and 30℃ respectively, and thesimilar results in 5% CO_2 or in normal atmosphere. The virus particles inculture fluid of infected cells were further eonfirmed by using ELISA andelectron microscopy, and it could infect healthy grass carp as well
出处
《病毒学杂志》
CSCD
1989年第3期315-319,共5页
关键词
草鱼
出血病病毒
生长特性
Grass Carp Hemorrhage Virus
Growth characteristic
Virus titer
