摘要
目的 :测定高良姜中总黄酮的含量 ,为其质量标准的制订提供依据。方法 :以乙醇提取药材 ,醋酸乙酯萃取 ,聚酰胺柱层析分离 ,紫外分光光度法测定高良姜中总黄酮的含量。结果 :高良姜中总黄酮的含量为 3.44% ,加样回收率为 96 .9% (RSD =2 .1% )。结论 :该法灵敏度高 ,稳定性好 ,可为制订高良姜的质量指标提供方法依据。
Objective:To Determine the total content of flavonoids in Alpinia officinarum Hance (AOH), in order to provide basis for working out the quality standard of the medicinal materials of AOH.Methods: Extracted the medicinal materials with ethanol, then extracted it with ethyl acetate, determined the total content of flavonoids in AOH by polyamide column chromatography-ultraviolet spectrophotometry. Results: The total content of flavonoids was 3.44%, the rate of recovery was 96.9% (RSD=2.1%). Conclusion: This method is accurate, stable and sensitive that may serve as a way of quality standard drawing up for the medicinal materials of AOH.
出处
《时珍国医国药》
CAS
CSCD
2001年第6期486-487,共2页
Lishizhen Medicine and Materia Medica Research
关键词
高良姜
总黄酮
紫外分光光度法
含量
测定
中药
Alpinia officinarum Hance
The total flavonoid
Ultraviolet spectrophotometry
Content determination
