摘要
本文报导了从大量抗HIV-1结构蛋白单抗和抗nef肽、nef蛋白单抗中发现的HIV-1及其nef蛋白存在有与正常人组织呈交叉反应的表位。这些单抗的特异性为抗HIV-1 p55+p24;抗gp160+gp120+gp41;抗p18+p27;抗nef蛋白(表位为由LHGM组成的四个氨基酸顺序)及抗nef蛋白肽(表位为由HPE或HPEY组成的氨基酸顺序)。它们与正常人扁桃体中的基质、血管内皮或平滑肌起反应。文中对AIDS病人自身免疫反应性进行了讨论。
We have produced 48 murin monoclonal anti-HlV-l proteins antibodiest 11 anti-nef peptide (C-terminal cosensus sequence) and 97 anti-nef+ P27 recombinant protein. From it 33 McAbs (1 anti-P24 + P55; 10 anti-P18 + p27; 2 anti-gp160 + EP120 + gp41; 6 anti-nef peptide (C-terminal cosensus sequence) 1 anti-nef peptide 20, 8 anti-nef pep-tide 20/21; 3 anti-nef peptide 21; 2 anti-nef peptide24/23) had crossreactivity with stroma, endothelium or smooth muscle of small blood vessel in normal human tonsil. Some epi-topes of the cross-reactivity were defined. They were LHGM and HPEor HPEY.
The significance and role in Multiple-Antigen-Mediated Autoimmunity (MAMA) in AIDS has been discussed.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1991年第3期147-150,165,共5页
Immunological Journal
关键词
HIV-1
NEF蛋白
单克隆抗体
艾滋病
Monoclonal antibody, Anti-HIV-1, Anti-nef protein, Anti-nef peptide cross-reaction, Epitope
