摘要
目的 : 研究了 Mg2 + 对氢过氧化物 ( H2 O2 和 t-丁基氢过氧化物 )诱导的人脐静脉内皮细胞脂质过氧化和抗氧化酶超氧化物歧化酶 ( EC- SOD)及含硒和不含硒两类谷胱甘肽过氧化物酶 ( Se- GSH- Px和 non- Se- GSH- Px)活性的影响。方法 : 用培养的人脐带内皮细胞以硫代巴比妥酸反应物 ( TBARS)法检测细胞脂质过氧化水平 ,邻苯三酚自氧化法测定 EC- SOD活性 ,DTNB直接法测定内皮细胞 Se- GSH- Px和 non- Se- GSH- Px活性。结果 : 1 .与 H2 O2 组相比 ,H2 O2 + Mg2 +各剂量组 TBARS显著下降 ,P<0 .0 5 ;t-丁基氢过氧化物 + Mg2 + 各剂量组与 t-丁基氢过氧化物组相比 TBARS无显著性差异。 2 .与空白对照组相比 ,0 .6mmol/L、1 .2 mmol/L、2 .4mmol/L补Mg2 + 组 SOD活性升高 ,具有显著性意义 ( P<0 .0 5 )。 3.与空白对照组相比 ,补 Mg2 + 各剂量组 Se-GSH- Px活性升高 ,P<0 .0 5 ;与 H2 O2 组相比 ,H2 O2 + Mg2 + 各剂量组 Se- GSH- Px和 non- Se- GSH-Px活性升高 ,具有显著性意义 ( P<0 .0 5 ,P<0 .0 1 )。结论 : Mg2 +能对抗内皮细胞脂质过氧化反应 ,并增强抗氧化酶的活性。
Objective:The effect of magnesium on lipid peroxidation of endothelial cells induced by H 2O 2 and t bultyl hydroperoxide and subsequent alterations of extracellular superoxide dismutase(EC SOD) and cellular selenium dependent and selenium independent GSH Px were studied. Methods:The extent of cellular lipid peroxides was determined by measuring the formation of TBARS, EC SOD by the method of pyrogallol auto oxidation, and GSH Px by the method of DTNB. Results:The results showed that the formation of TBARS was significantly reduced in the group of H 2O 2+Mg 2+ at doses of 0.3, 0.6, 1.2,and 2.4 mmol/L compared to the group of H 2O 2(P<0.05).In contrast, the formation of TBARS did not show significant differences between the group of t butyl hydroperoxide and the group of t butyl hydroperoxide+Mg 2+ .Compared with the group of H 2O 2, the activity of EC SOD, GSH Px with and without selenium significantly increased in the group of H 2O 2+Mg 2+ at all doses(P<0.05,P<0.01,respectively). Conclusion:The results show that magnesium protects endothelial cells from lipid peroxidation and reinforces the activities of antioxidant enzymes..
出处
《营养学报》
CAS
CSCD
北大核心
2001年第3期250-253,共4页
Acta Nutrimenta Sinica
基金
博士点基金 ( No.995 8)
关键词
镁
人脐静脉内皮细胞
脂质过氧化
抗氧化酶
magnesium
human umbilical vein endothelial cells
lipid peroxidation
antioxidant enzymes
