摘要
目的 探讨碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)及肥大细胞在翼状胬肉形成和发展过程中的作用。方法 对 17例初发性翼状胬肉、6例复发性翼状胬肉及 6例正常球结膜组织标本行bFGF和肥大细胞类胰蛋白酶单克隆抗体检测 ,观察肥大细胞浸润数量及其bFGF表达的情况。结果 初发性翼状胬肉组织标本中 ,上皮细胞层、血管壁及结缔组织中可见少量细胞有bFGF阳性表达 ;复发性翼状胬肉组织标本中 ,bFGF阳性表达较初发性翼状胬肉组织强。初发性和复发性翼状胬肉组织标本中肥大细胞浸润数量分别为 (4 5 4 7± 5 5 0 )及 (4 8 83± 3 19)个细胞 / 5个视野 ,与正常结膜组织 (4 2 4± 2 36 )个细胞 / 5个视野比较 ,差异有显著性 (F =2 0 0 312 8;q =2 6 6 76 2 ,2 3 734 1;P <0 0 5 )。翼状胬肉组织中 ,几乎所有染色阳性的肥大细胞形态、分布均与结缔组织中bFGF染色阳性的细胞相同 ,而结缔组织内bFGF染色阳性的细胞形态、分布及数量同肥大细胞 ,占 (87 5 4± 3 6 0 ) %。结论 翼状胬肉组织标本中浸润的肥大细胞均可见bFGF的阳性表达。翼状胬肉组织上皮细胞、血管壁及浸润的肥大细胞bFGF阳性表达增加 ,可能与翼状胬肉的形成和发展有关。
Objective To examine the expression and distribution of basic fibroblast growth factor (bFGF) and mast cells in pterygium, and evaluate its effects in the pterygium formation and progression. Method The expression of bFGF and mast cell tryptase in 17 primary pterygia, 6 recurrent pterygia and 6 normal conjunctival specimens were studied. The mast cell count and bFGF expression situation were observed. Results The bFGF was specifically localized in the epithelium, blood vessels and a subset of connective tissue cells. The bFGF expression was increased in the recurrent pterygium. The numbers of infiltrating mast cells (five 400×sights) were (45.47±5.50) cells and (48.83±3.19) cells in the primary and recurrent pterygium respectively. In the comparisons between the cells in the pterygium (primary and recurrent) and (4.24±2.36) cells in the normal connective tissue, there were significant differences (F=200.312 8; q=26.676 2, 23.734 1; P<0.05). The shape and distribution of all the tryptase-positive cells (mast cells) in the pterygium tissues were similar to that of the cells with bFGF expression in the connective tissue. And the majority of bFGF-positive cells (87.54±3.60)% were similar to that of mast cells in the connective tissue. Conclusions All infiltrating mast cells in pterygium have bFGF-positive expression. The bFGF expression is increased in the epithelium, blood vessels and infiltrating mast cells of the pterygium, and may contribute to the formation and progression of a pterygium.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2001年第6期455-457,T005,共4页
Chinese Journal of Ophthalmology
