摘要
[目的 ]建立挥发性物质体外致突变的定量检测方法。 [方法 ]运用物质相平衡的原理 ,利用一组平衡瓶和一组平衡参照瓶及顶空气相色谱技术 ,检测挥发性物质培养基 /气的分配系数。设计一种新型的挥发性物质体外致突变密闭检测装置 ,定量检测挥发性物质的致变性。 [结果 ]通过 9种挥发性物质的分配系数测定 ,证明挥发性物质在达到培养基 /气平衡后 ,在两相中的浓度可保持不变 ,且可通过计算求出浓度。从而解决了培养基中挥发性物质的浓度定量问题。建立了特制的专供气体物质和挥发性物质进行Ames试验的新的容器装置。 [结论 ]沸点在 10 0℃以下的挥发性物质 ,必须用密闭容器装置检测其致变性 ,沸点在 10 0～ 2 0 0℃的 ,容器装置法亦是首选的检测方法。容器法试验中挥发性物质在培养基中的浓度可通过分配系数测定确定。
Objective] To establish a method for quantitative determining of the mutagenicity of volatile substances in vitro . [Methods] Based on the phase equilibration principle,media/air partition coefficients of volatile substances were measured using a series of equilibration and reference bottles and headspace gas chromatography technique. A new sealed device was designed for quantitative detection of mutagenicity of volatile substances in vitro ,with this device the mutagenicity of volatile substances was determined. [Results] The determination of the media/air partition coefficients demonstrated that the concentration of volatile substance in the two phases was stable after equilibration,and so the concentrations can be calculated. The new special sealed device of Ames test for gas and volatile substances was elaborated. [Conclusion] When Ames test for volatile substances with the boiling point below 100℃ are conducted,the sealed device should be used; when volatile substances with the boiling point from 100℃ to 200℃ are tested,the sealed device is still useful and of first choice. The concentration of volatile substance in media can be quantified based on partition coefficients in the test using the sealed device.
出处
《劳动医学》
北大核心
2001年第3期135-138,共4页
基金
上海市科学技术发展基金 (上海市卫生局 ) (90 4 2 4 )
