摘要
本文采用荧光定量聚合酶链反应 (FQ PCR)和ELISA两种方法同时检测了 310份血清 ,并对结果进行了对比分析 ,结果显示 :80例HBsAg (+)、HBeAg (+)、HBcAg (+)组的血清HBVDNA检出率为 92 5 % (74例 ) ,平均拷贝数为 4 10× 10 10 /ml,6 8例HBsAg (+)、HBeAb (+)、HBcAb (+)组血清HBVDNA检出率为32 35 % (2 2例 ) ,平均拷贝数为 1 31× 10 9/ml,70例HBsAg (+)、HBeAb (+)组血清HBVDNA检出率为45 71% (32例 ) ,平均拷贝数为 4 0 8× 10 8/ml;32例HBV M全阴性组血清HBVDNA检出率为 6 .6 7% (2例 ) ,平均拷贝数为 1 5 4× 10 8/ml。结果表明 :HBV -M阴性的病人也可能有HBVDNA阳性 ,因此为临床提供HBV感染、复制及传染性的判断以及指导治疗 。
clinical serum samples were tested by Fluorescence quantitative PCR assay (FQ PCR),using ELISA as contrast.In 74 HBsAg+/HBeAg+/HbcAb+samples,FQ PCR results were positive,the average level of HBV DNA was 4 16×10 10 ml with a positive rate of 92 50%.In 22 HBsAg+/HbeAb+/HbcAb+samples the average level was 1 31×10 9/ml with a positive rate of 32 35%.In 32 HBsAg+/HBcAb+samples the amount was 4 04×10 8/ml with a positive rate of 45 71%.In 2HBV M(-)sample,the amount of HBV DNA was 1 54×10 8/ml with a positive rate of 6 67%.The results showed that HBV DNA could be positive by FQ PCR,even it may be negative for HBV M,So FQ PCR can be used as another good monitor the state of HBV infection and complication.
出处
《华西医学》
CAS
2001年第3期332-334,共3页
West China Medical Journal
