摘要
目的 :探讨外周血中AFPmRNA检测对原发性肝癌 (以下简称肝癌 )高危人群监测的价值。材料和方法 :采取AFP升高 2次以上的慢性肝病患者 6 3例、12例肝癌、2 1例肝癌术后患者静脉血 5ml,用RT -PCR进行AFPmRNA检测。结果 :AFP定量与AFPmRNA阳性率无显著关系 (P >0 .0 5 ) ,AFP进行升高组、持续升高组和波动组间AFPmRNA阳性率无显著关系(6 4.71%、40 %、40 .5 8% ,P >0 .0 5 ) ,AFP进行升高组、持续升高组发癌率为 5 8.82 % (10 / 17) ,30 % (3/ 10 )显著高于AFP波动组 4.35 % (3/ 6 9,P <0 .0 1)。AFPmRNA阳性检出肝癌 41.6 7% (5 / 12 ) ,高危结节 6 0 % (6 / 10 )、肝癌术后复发 10 0 % (4/ 4)。AFP大于 2 0 0 μg/L能检出 5 8.82 % (10 / 17)肝癌和 2 5 % (1/ 4)的术后复发灶 ,AFP大于 2 0 0 μg/L、AFPmRNA阳性结合影像检测能检出 91.6 7% (11/ 12 )的肝癌和 10 0 % (4/ 4)的术后复发灶。结论AFP进行和持续升高是肝癌发生的高危人群 ,AFPmR NA阳性也是肝癌发生的高危人群 ,AFPmRNA能检测部分AFP阴性的肝癌患者 ,因此 ,AFP。
Objective: To study the value of hAFPmRNA in peripheral blood for detecting HCC in high risk population. Methods: 5ml sample of peripheral blood were collected from 63 patients with chronic liver diseases, with 12 of HCC and 21 of curative HCC resection, hRNAmRNA was detected by reverse-transcriptase polymerase chain reaction. Results: There was no significant correlation between quantitation of serum AFP and frequency of hAFPmRNA positivity( P >0.05).The frequencies of positivity were 64.71% in group with continuous elevated serum AFP values,40% in group with progressive elevated serum AFP ,and 40.58% in group with fluctuating serum AFP . These results showed no significant correlation.The incidence of HCC was significantly more frequent in former two groups (58.82%) than that in the latter( P <0.01). 10 of 17(58.82%) patients with HCC and 1 of 4(25%) with recurrent HCC were detected by serum AFP larger than 200μg/L,5 of 12(41.67%) patients with HCC, all the recurrent HCC(4 cases) and 6 of 10(60%) with high risk nodular were detected by AFPmRNA. Combination of AFP, AFPmRNA and ultrasonography , MRI or CT,showed the detectivity to be 11 of 12 (91.67%) in HCC and 4 of 4(100%) in recurrent HCC. Conlusion: Serum AFP continuously and progressively increasing is the marker of presence in high risk population and AFPmRNA positivity is aslo an important one. AFPmRNA positive could reveal some HCC in patients with negative serum AFP. Combination of imaging examinations and tests of serum AFP and AFPmRNA could raise the detectivity of small HCC.
出处
《中国临床医学》
2001年第4期313-315,319,共4页
Chinese Journal of Clinical Medicine
基金
江苏省青年科研基金资助 编号BQ96 0 44
关键词
AFPMRNA
小肝癌
外周血
检测
Alpha-fetoprotein mRNA Small hepatocellular carcinoma
