摘要
目的 副流感病毒的分离、鉴定。方法 应用鸡胚和人胚肺成纤维细胞突变株分离病毒 ,用多株流感国际和国内代表株抗血清进行血凝抑制交互试验 ,用 4类主要呼吸道病毒单克隆抗体间接免疫荧光法做特异性交叉反应鉴定。结果 分离株在鸡胚培养中有较高的血凝效价 ,但与流感病毒甲、流感病毒乙均不发生血抑反应 ,而与副流感病毒2型单克隆抗体发生较强免疫荧光反应 ,在人胚肺成纤维细胞突变株培养出现明显细胞融合病变。结论 分离株虽具有高滴度血凝效价 ,但不是流感病毒 ,根据与副流感病毒 2型单克隆抗体可以发生较强反应和在人胚肺成纤维细胞上的特征性细胞病变 ,确认为副流感病毒 2型。
Objective To isolate and identify parainfluenza virus.Methods Viruses were isolated by using chicken embryo and mutant lung fibroblast of human embryo(Z-HL-16C).The viruses isolated were assayed by hemagglutination test(HA),hemagglutination inhibition test (HAI)with several international and national influenza strains,vitus antigen slides of them were also assayed by specific cross test with McAb against 4 main respiratory track viruses.Results HA titer of two isolated strains was high,The results of HAI test were nagative when assayed with anti-sera of FluA and FluB virus,but strong fluorescence were obtained when antigen slides of two isolated strains were tested with PIV-2 McAb,pathological changes of cell fusion were obtained in Z-HL-16C.Conclusions The virus isolated were not influenza virus,and identified as PIV-2 by results of McAb fluorescent staining and pathologocal changes in Z-HL-16C.
出处
《预防医学情报杂志》
CAS
2001年第5期334-335,共2页
Journal of Preventive Medicine Information
关键词
副流感病毒
血凝抑制试验
呼吸道病毒单克隆抗体
分离
鉴定
Parainfluzenza virus
Hemagglutination inhibition test
McAb against 4 main respiratory track viruses
