摘要
目的 确立激光诱发自体荧光 (LIAF)光谱区分肺癌组织和正常肺组织的判断依据。探讨应用激光诱发自体荧光 (LIAF)光谱方法诊断肺癌的可行性。方法 收集肺癌手术标本 42例 ,使用三倍频YAG激光 (波长 35 5nm)和光学多通道分析仪 (OMA)测定肺癌标本的激光诱发自体荧光光谱 ,根据正常肺组织和肺癌组织的激光诱发自体荧光光谱特征规律 ,寻找能够区分两者的光谱差异 ,得出判断依据 ,并与病理检查结果比较。结果 (1)正常肺组织在 5 80nm与 6 0 0nm的荧光强度比为 0 842± 0 15 8;肺癌为 1 2 6 9± 0 147(P <0 0 0 1)。 (2 )肺癌阳性判断标准域值 :荧光强度比值判据为I580nm/I60 0nm≥ 1 0 73;与病理检查结果比较 ,符合荧光强度比值判据的判断敏感性为 93.2 % ,特异性为 88.1% ,阳性预测值为 89.6 % ,阴性预测值为 92 .2 %。
Objective To obtain the criterion of Laser induced autofluorescence (LIAF) spectroscopy in the differentiation of normal lung tissue and lung cancer and study the feasibility of LIAF spectroscopy in the diagnosis of lung cancer. Methods The LIAF spectra of normal lung and lung cancer in 42 surgical specimens have been measured with a detecting system which consists of an YAG laser(wavelength 355 nm) and an optical multichannel analyzer(OMA). Spectroscopic differences between normal lung and cancerous tissues have been found which could be used as a criterion to distinguish from them. The pathological examinations were done to compare with the criterion. Results (1) In the normal lung tissue spectrum, there were two subsidiary maxima located clearly at 560 and 600nm and the cancer tissue spectrum shows an almost structureless and smoother profile with no subsidiary maxima. The mean fluorescence intensity ratio values at 560nm and 600nm were 0 842±0 158 for normal lung tissue and 1 269±0 147 for lung cancer (P<0 001). (2) The criterion for detection of lung cancer: the fluorescence intensity ratio of I 580nm /I 600nm was larger or equal to 1 073. Compared with the pathological findings, the criterioncan differentiate lung cancer from normal lung tissues with the sensitivity, specificity, positive predictive value and negative predictive value of 93 2%, 88 1%, 89 6% and 98 3% respectively. Conclusion It is feasible to differentiate lung cancer from normal lung tissue and normal bronchus with the use of LIAF spectroscopy.
出处
《中国激光医学杂志》
CAS
CSCD
2001年第4期214-217,共4页
Chinese Journal of Laser Medicine & Surgery
