摘要
目的 :探讨人乳头状瘤病毒 HPV感染及抑癌基因 p5 3、增殖细胞核抗原 PCNA在维吾尔族口腔鳞癌中的异常表达与肿瘤病理生物学特征间关系及临床意义 ,为维吾尔族口腔鳞癌有效防治提供实验科学依据。 方法 :(1)应用免疫组化 L SAB法检测维吾尔族口腔鳞癌 p5 3蛋白及 PCNA的表达及变化。 (2 )用 PCR技术检测HPV感染率。结果:p5 3及 PCNA在维吾尔族口腔鳞癌中阳性率分别为 71.2 %、84.8% ,而在口腔正常组织中阳性率分别为 8.3%、16 .7% ,肿瘤与正常组织间差异有统计学意义 (P <0 .0 5 )。HPV在维吾尔族口腔鳞癌中阳性率为42 .4% (2 8/ 6 6 ) ,而在口腔正常组织中阳性率为 8.3% (1/ 12 ) ,两者间差异有统计学意义 (P <0 .0 5 )。结论:(1)抑癌基因 p5 3,增殖细胞核抗原 PCNA在细胞内的过度表达可能在口腔鳞状上皮组织良性病变的恶性转化过程起关键性的作用 ,可对良恶性病变从分子水平进行评估 ,并可作为早期诊断及临床治疗维吾尔族鳞癌的分子生物学指标。 (2 ) HPV16、18型感染与口腔鳞癌的发生密切相关 。
Objective: Alterative expression of p53?PCNA and infections of HPV in tumor tissues of oral squamous cell carcinoma in Uygur patients related to the biopathology and clinical significance was studied. It may provide scientific basis for effective prevention and cure of this tumor. Methods: (1) Expression of p53 and PCNA in tumor tissues of 56 Uygur patients were detected by immunohistochemistry (LSAB). (2)HPV infection in oral squamous cell carcinoma tissues was detected by PCR. Results: The positive frequency of p53 and PCNA was 71.2% and 84.8% in cancer tissues and 8.3% and 16.7% in normal oral mucous tissues, respectively. It revealed significant difference (P<0.05). The positive frequency of HPV 16,18 in OSCC was 42.4%, it revealed more higher than in normal oral mucous tissues (8.3%) (P<0.05). Conclusion: (1) Immunohistochemical examination of the alterative expression of tumour suppressor gene p53, proliferating cell nuclear antigen (PCNA), may offer useful molecular index for the early diagnosis and clinical therapy of OSCC. (2)The results suggest that HPV 16,18 infection may involve an important role in carcinogenesis of OSCC.
出处
《新疆医科大学学报》
CAS
2001年第3期200-202,共3页
Journal of Xinjiang Medical University
基金
新疆医科大学一附院科研启动基金 (2 0 0 0 -YFY-15 )
