摘要
利用定点突变技术将人干扰素 β第 17位半胱氨酸编码序列突变为丝氨酸 (IFN βser17) ,DNA序列分析证明了其核苷酸序列的正确性 ,并在大肠杆菌TAP10 6中获得高效表达 ,IFN βser17表达量达 2 0 % ,用Westernblot得到单一条带 ,细胞病变抑制法测定其比活性达 (2～ 3)× 10 7U mg。INF βser17的比活性和稳定性均超过天然人IFN β。
In the natural interferon-beta, the residue Cys at the position 17 was replaced with Ser by sits-specific mutagenesis. Analysis of DNA sequencing demonstrated that IFN-βser17 has the high expression in E.coli TAP106, the expression level was more than 20%. The result of Western blot showed that the expression protein was correct. By the way of WISH-VSV cell inhibition, relative activity was about (2～3)×10 7 U/mg. The activity and stability of INF-βser17 were better than the natural interferon-beta.
出处
《生物技术通讯》
CAS
2001年第3期202-204,共3页
Letters in Biotechnology
