摘要
目的 研究不同维生素A、锌营养水平与小鼠胚胎HOXC4(3 5 )基因表达的相关关系。方法 昆明种雌鼠随机均分为 3组 :缺乏组喂饲无维生素A和低锌饲料 ;补充组 :开始时饲料同缺乏组 ,母鼠受孕第 7天改为富含维生素A和锌的饲料 ;正常组自始至终喂饲正常饲料。动物喂养至出现明显维生素A、锌缺乏症状后进行交配。受孕第 12天取出相应数量孕鼠处死 ,取样测生化指标 ;取胚胎测定HOXC4(3 5 )基因mRNA表达量。结果 缺乏组、补充组和正常组母鼠血清维生素A水平均值分别为 0 44 μmol/L、1 0 3μmol/L和 1 40 μmol/L ,股骨锌含量均值分别为 12 4 3mg/kg、15 2 1mg/kg和 193 8mg/kg,差异有显著性。胚胎HOXC4(3 5 )基因表达也显著升高。结论 小鼠机体维生素A和锌营养水平与胎鼠HOXC4(3 5 )基因表达呈高度正相关 ,各项指标相关系数为 0 78～ 0 99。
Objective Correlation between nutrient levels of vitamin A and zinc and HOX C4 (3 5) gene expression in mice embryos was studied Methods The Kunming female mice were divided three groups: deficient group (DG) fed with fodder deficient of vitamin A and zinc, replenished group (RG) fed with fodder deficient of vitamin A and zinc until the 7th day after conception and then fed with fodder rich in vitamin A and zinc, and normal group (NG), fed with normal fodder After obvious symptoms of vitamin A and zinc deficiency appeared, the mice were mated On the 12th day after conception, pregnant mice were killed and their levels of vitamin A and zinc were determined and the content of mRNA of HOX C4 (3 5) were determined for the embryos with situ hybridization Results The level of vitamin A was 0 44 μmol/L, 1 03 μmol/L and 1 40 μmol/L, respectively and that of zinc 124 3 mg/kg,152 1 mg/kg and 193 8 mg/kg in DG, RG and NG, respectively The mRNA of HOX C4 (3 5) gene expression in mouse embryos increased significantly in DG, RG and NG ( P <0 05) Conclusions Levels of vitamin A and zinc in the body of pregnant mice positively correlated significantly with their HOX C4 (3 5) gene expression, with coefficients of correlation of 0 78 to 0 99
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2001年第6期378-380,共3页
Chinese Journal of Preventive Medicine
基金
"九五"国家科技攻关项目 ( 96 90 4 0 6 0 6 )
