摘要
目的 :研究血管生成抑制物 TNP- 470对肺高转移性涎腺腺样囊性癌 (ACC- M)在裸鼠体内生长和转移的抑制作用 ,探讨其作用机制。方法 :46只接种 ACC- M细胞的裸鼠被随机分为 15、30、6 0 mg/ kg用药组及对照组。用药组在经尾静脉接种肿瘤细胞后第 7天分别给予皮下注射不同剂量的 TNP- 470 ,隔日 1次 ,共 8次 ;对照组同期给予皮下注射等量溶剂。接种 6周后处死实验动物。观察发生肿瘤肺转移的裸鼠数量 ,称量转移肺湿重 ,统计肺转移灶数目和肺转移灶面积 ,计算肺转移灶面积比。检测 ACC- M肺转移灶肿瘤微血管密度 (MVD)、肿瘤细胞增殖指数 (PE)及凋亡指数 (AI)。结果 :30和 6 0 mg/ kg组裸鼠肿肺转移率、转移灶数量、转移肺湿重、转移灶面积以及转移灶面积比均低于对照组 ,统计学分析有显著性差异 (P<0 .0 5 )。TNP- 470治疗组肿瘤细胞 PI与对照组无显著差异 ,但其 AI均较对照组增高 ,差异明显(P<0 .0 5 )。结论 :TNP- 470具有抗 ACC- M裸鼠肺转移作用 ,30和 6 0 mg/ kg组用药疗效较为理想。抑制肿瘤血管生成 ,诱导 ACC- M细胞凋亡可能与 TNP- 470抗 ACC-
Objective:To observe the inhibitive effects of the angiogenesis inhibitor,TNP 470,on the growth and lung metastases of highly lung metastatic salivary adenoid cystic carcinoma(ACC M)in nude mice,to investigate the mechanisms of TNP 470 against ACC M in vivo.Methods:The nude mice were randomized into three treatment groups(15,30 and 60 mg/kg)and a control group.From the second week,TNP 470 was administered subcutaneously every other day for two weeks.The mice were sacrificed at the seventh week after implantation.The lung metastasis rates and weights of mice were detected.The numbers and area of metastases and ratio of area of metastases were analyzed.The microvessel density(MVD),cell proliferation index(PI)and apoptosis index (AI)of the ACC M lung metastases were observed.Results:Compared to the control,the lung metastasis rates,the weight ,the numbers,the area of metastases,the ratio of area and the MVD of metastases were significantly reduced in mice treated with 30 and 60mg/kg TNP 470 (P<0.05).The AI of ACC Mcell was higher in the experimental groups (P<0.05).Conclusions:TNP 470 suppressed the growth and lung metastases of ACC M in vivo.The inhibitive effects of 30 and 60mg/kg TNP 470 were better.We suggest that inhibiting angiogenesis,inducing apoptosis are the mechanisms of TNP 470 on the growth and metastases of ACC M in nude mice.
出处
《耳鼻咽喉（头颈外科）》
2001年第6期355-358,共4页
Chinese Arch Otolaryngology-Head Neck Surg
