摘要
目的研究胰岛素B链羧端的自由度对于胰岛素与受体相互作用的影响。方法在人胰岛素B链羧端 β转角区回折点B1 9与B2 0 之间插入一个Gly。结果B1 9 Gly B2 0 人胰岛素原的表达产物占细胞总蛋白量的 3 0 % ,B1 9 Gly B2 0 人胰岛素的受体活性是标准猪胰岛素的 12 1%。结论以较高的受体结合活性获得了高纯度的B1 9 Gly B2 0 人胰岛素。
PurposeThe aim is to study the effect of increased flexibility of C terminal of insulin B chain on its receptor binding activity. MethodsA Gly was inserted between B 19 and B 20 of turn in of insulin B chain β hope of providing more flexibility to its C termina. ResultsThe expression level of B 19 Gly B 20 human prolnsulin accounted for 30% of total bacterial proteins. B 19 Gly B 20 human insulin′s receptor binding activity was 121% as compared with standard porcine insulin.ConclusionPurified B 19 Gly B 20 human insulin was obtained by the common methods and its receptor binding activity was high.
出处
《中国生化药物杂志》
CAS
CSCD
2001年第5期234-236,共3页
Chinese Journal of Biochemical Pharmaceutics
基金
北京大学蛋白质工程及基因工程国家重点实验室资助项目
