摘要
目的 探讨新生儿筛查滤纸干血样法检测过程中 ,可能影响促甲状腺素 (TSH)及苯丙氨酸 (phe)测定结果的因素 ,以建立有效的改进措施。方法 观察采血时间等因素对酶免疫标记法(BA ELISA)和细菌抑制法结果的影响 ,观察其变化规律。结果 新生儿筛查试验影响因素众多 ,TSH测定 :72h后采血结果稳定 ,季节间TSH水平有差异 ,应选择不同的适宜的筛查临界值 ;滤纸血样自然干燥易于洗脱 ;于干血样 1/ 4处取样结果近于靶值 ;BA ELISA洗脱 4～ 5h效果较好。phe测定 :72h后采血可减少假阳性 ;制板温度以 5 5～ 6 0℃为宜 ,加血样间距不宜过密。结论 采血时间、临界值、血片处理等因素影响筛查结果 ,选择最佳实验条件 。
Objective To investigate factors influencing thyroid stimulating hormone (TSH) and phenylalanine (phe) assay in dry blood spots on filter paper. Methods The influence on enzyme linked immunoassays and bacterial inhibition assay were studied so as to control their changes. Results Many influencing factors were noted in newborn screening assay. TSH results were stable if specimens were collected form newborns 72 hours after delivery. Different results were obtained when TSH was tested in different seasons. Specimens were air dried at room temperature before transportation. To reduce false positive phe result, specimens were collected from newborns 72 hours after delivery. The phe result was very stable with a plating temperature of 55 60℃ and speradical distribution of blood spots . Conclusion To assure the quality of screening the time of specimens collecting, different cut off, blood spots disposition and best condition should be controlled.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2001年第6期339-341,共3页
Chinese Journal of Laboratory Medicine
