摘要
文中研究利用UV、DES及Co60 -γ对栖土曲霉进行多因子复合诱变处理 ,在以蚕蛹为主要原料的固体培养基上进行培养 ,选育出了一株生长繁殖速度快、孢子丰富且蛋白酶活高的变异菌株DC -7。其蛋白酶活达 86 2 8 9u/g (干曲 ) ,较出发菌株AS 3 374酶活 40 12 3u/g (干曲 )提高了 115 %。此外 ,其遗传稳定性也较好。
Aspergillus.terricola is mutated complexly by UV,DES and 60 Co γ,then cultured on the solid medium with main material of silkworm chrysalis.A mutant of DC-7 is scereened, which has characteristics of fast growth rate,spore riches and high protease activity.Its protease activity is up to 8628.9 units per gram dry leaven.Moreover,its genetic stability is very good.
出处
《中国酿造》
CAS
北大核心
2001年第6期13-16,共4页
China Brewing
关键词
生产菌株
诱变选育
蚕蛹
酱油酿制
Aspergillus·terricola
protease
induced mutation
silkworm chrysalis
