摘要
目的 研究银屑病患者体内糖皮质激素水平及其受体基因表达的变化 .方法 用酶免疫法测定了 2 2例银屑病患者、15例临床治愈的银屑病患者和 2 0例正常人对照组血清糖皮质激素水平 ,用 RT- PCR方法检测 12例银屑病患者和10例正常人对照血白细胞糖皮质激素受体 m RNA的表达水平 .结果 银屑病患者组血清糖皮质激素水平 (0 .5 8± 0 .0 7μmol· L- 1 )高于银屑病临床治愈组 (0 .49± 0 .11μmol· L- 1 )和正常对照组 (0 .48± 0 .10 μmol· L- 1 ) ,差异有极显著性(P<0 .0 1) ,银屑病患者组血白细胞糖皮质激素受体 m RNA表达明显低于正常对照组 (P<0 .0 1) .结论 银屑病患者体内糖皮质激素浓度不低于正常水平 ;银屑病患者组血白细胞糖皮质激素受体 m RNA表达低于正常水平 ,提示银屑病患者体内促肾上腺皮质激素释放激素 -糖皮质激素 -糖皮质激素受体系统平衡失调 。
AIM To study the change of glucocorticoid level and the expression of its receptors in the blood of psoriatic patients. METHODS We detected the level of serum glucocorticoid of 22 psoriatic patients, 15 patients who had been clinical cured and 20 normal controls by enzyme immunoassay (EIA); the glucocorticoid receptors mRNA expression levelsin blood leucocytes of 12 psoriatic patients and 10 normal controls were measured by semi quantitative reverse transcription polymerase chain reaction (RT PCR). RESULTS Level of serum glucocorticoid was higher in psoriatic patients (0.58± 0.07 μmol·L -1 ) than in the normal controls (0.48± 0.10 μmol·L -1 ) and patients who had been cured (0.49±0.11 μmol·L -1 ) ( P <0.01); the glucocorticoid receptor mRNA expression levels in blood leucocytes of psoriatic patient group were significantly lower than that of the normal control group ( P <0.01). CONCLUSION Glucocorticoid level is not lower in psoriatic patients. The glucocorticoid receptor mRNA expression levels in blood leucocytes of psoriatic patient group are lower than may exist normal level, which suggests there a dysregulated glucocorticoid, glucocorticoid receptor and corticotropin releasing hormone system in psoriatic patients, and they may play a role in the pathologic course of psoriasis.
出处
《第四军医大学学报》
北大核心
2001年第22期2091-2093,共3页
Journal of the Fourth Military Medical University
关键词
银屑病
受体
糖皮质激素
基因表达
psoriasis
receptors, glucocorticoid
gene expression
glucocorticoids
