摘要
目的 探讨异体腹直肌前鞘细胞外基质框架 (ASECM )作为疝修补材料的效果。方法 12只犬随机分为 2组 :(1)ASECM修补组 (实验组 ) ,6只 ;(2 )人工材料修复组 (对照组 ) ,6只。手术于腹直肌左右侧腹壁制成一 3cm× 5cm的缺损区 ,右侧缺损区实验组用ASECM行修补 ,对照组用人工材料补片修补。左侧缺损区不作修补用作自体对照。两组分别于术后2 ,8,16周取材行光镜、免疫组化、扫描及透射电镜检查。结果 两组动物左侧腹壁缺损处 2d后出现腹壁疝 ,而修补区均未出现腹壁疝。实验组光镜检查显示 ,术后 2～ 8周ASECM中长入的宿主成纤维细胞逐渐增多 ,术后 16周时成纤维细胞数量减少。各时期均无明显的炎性细胞浸润。实验组电镜检查术后 16周修补局部基本为排列整齐的细胶原。对照组光镜检查显示 ,术后 2～ 16周时可见由少量～大量的成纤维细胞附于材料层面上 ,但人工材料网丝间隙之间未见明显的成纤维细胞生长。且在各个时期均有炎性细胞浸润。术后 16周对照组电镜检查时 ,成纤维细胞生长出的突起包饶材料表面 ,类似宿主对异物组织的包裹反应 ,且人工材料补片未与宿主组织融合。免疫组化检查显示为I ,III型胶原。结论 ASECM为机体提供了一良好的供宿主成纤维细胞“爬行替代”的框架 。
Objective To study the effect of allodecedllular sheath extracellular matrix(ASECM) for repairing of the hernia. Methods 12 dogs were randomly divided into two groups:(1)ASECM repairing group (ASECM group), 6 dogs; (2) artificial materia (AM) repairing group (Control group), 6 dogs. Abdominal wall defect of 3cm×5cm area was made on both of right and left abdomen in ASECM group and control group. The defect of left abdomen was not repaired as a self-control. The defect of right abdoment was repaired by ASECM in ASECM group , and by AM in control group . Microscopically, imunohistochemical study and electroscopy were performed 2,8,16 weeks after operation in both ASECM group and control geoup. Results A hernia could be seen on the defect of left abdominal wall in both groups 2 days after operation, but no hernia was found on the defect of right abdominal wall. In ASECM group , microscopy showed that the number of fibroblast cells (FBC) growing in the ASECM increased graduately 2～8 weeks after operation, but decreased 16 weeks after operation, and no inflammatory cells infiltration was seen at any time;electroscopically, the ASECM was filled with clear ranked thin collegen 16 weeks after operation. Contrarily, in control group, microscopy showed that the amount of covering the superficialness of AM increased graduately 2～8 weeks after operation, but not many FCs could be seen inside the AM, and inflammatory cells infiltration was found at every time postoperativly. Electroscopically, FCs grew to cover the superficialness as reaction of host to the foreign body ,and the AM had not merged with host tissues. Immuohistochemical study showed that there were typy I and III collegen presented in the repairing materias. Conclusions ASECM can provide a fram for the host FCs growth, So the ASECM can be mixed together with host tissues to provide a good intensive potency against hernia recurrence, and ASECM might be an ideal tissue-engineering materia for repairing hernia.
出处
《中国普通外科杂志》
CAS
CSCD
2001年第6期501-505,共5页
China Journal of General Surgery
关键词
移植
异种
腹直肌前鞘细胞外基质
对比研究
腹部疝
HERNIA, VENTRAL/surg
TRANSPLANTATION, XENOGENEOUS
ALLODECELLULAR SHEATH EXTRACELLULAR MATRIX
SURGICAL MESH
COMPARATIVE RESEARCH
