摘要
目的 为头孢噻肟钠在体内的血药浓度分析提供检测方法。方法 采用HPLC测定头孢噻肟钠血药浓度 ,4 .6mm×2 50mm ,1 0 μm ,YWG C18色谱柱 ,以 3 ,5 甲苯二酚为内标 ,流动相为 0 .0 1mol·L- 1磷酸二氢钠 甲醇 ( 75∶2 5) ,用氨水调 pH4 .5,检测波长 2 54nm。结果 头孢噻肟钠线性范围为 1 0～ 50 0 μg·mL- 1(r =0 .9986)最低检测浓度为 0 .5μg·mL- 1,平均回收率为 ( 92 .95± 0 .62 ) % ;日内、日间RSD均小于 2 %。结论 本方法具有快速、简便、灵敏、准确等特点 ,适合于头孢噻肟钠血药浓度测定。
OBJECTIVE: To determine the concentrations of cefotaxime sodium in serum by HPLC method. METHODS: The 4.6 mm × 250 mm, 10 μm, YWG-C18 column was used. 0.01 mol&bullL-1 Sodium dihydrogen phosphate-methanol (75:25) was selected as the mobile phase and Orcinol monohydrate was used as the internal standard. The detection wavelength was at 254 nm. RESULTS: The calibration curves were linear over the range of 10 ∼ 500 μg&bullmL-1 (r = 0.998 6) and the detection limit was 0.5 μg&bullmL-1, where the average recovery of the method was (92.95 ± 0.62)%. The RSD of intraday and interday variation were all less than 2%. CONCLUSION: This method appeared to be stable, simple, accurate and reliable. The technique was useful for determining the concentration of cefotaxime sodium in serum.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2001年第10期697-698,共2页
Chinese Pharmaceutical Journal
关键词
头孢噻肟钠
高效液相色谱法
血药浓度
Blood
Calibration
High performance liquid chromatography
Pharmacokinetics
