摘要
为了建立豚草花粉变应原致过敏性鼻炎的实验动物模型 ,将健康豚鼠 42只 ,随机分为致敏组 ( n=3 2 )和对照组 ( n=1 0 )。致敏组经腹腔、四肢皮下注射豚草花粉变应原免疫 4周后 ,鼻腔滴入豚草花粉变应原 ;对照组以生理盐水代替变应原。结果致敏组 2 8只出现鼻痒、喷嚏、流清涕等过敏性鼻炎的临床体征 (评分 >5分 ) ,鼻腔分泌物涂片见大量嗜酸性粒细胞 ,以及鼻粘膜水肿、粘膜嗜酸性粒细胞和肥大细胞浸润等组织学变化 ,而对照组无上述变化 ( P<0 .0 0 1 )。本实验建立的豚草花粉变应原致过敏性鼻炎的实验动物模型 ,为过敏性鼻炎 ,特别是豚草花粉变应原所致的过敏性鼻炎的诊断。
To establish an allergic rhinitis model induced by ragweed pollen allergen, guinea pigs(n=42) were divided into a sensitized group(n=32) and an unsensitized control group(n=10). Sensitized groups were immunized intraperitoneally and intracutaneously with ragweed pollen allergen for 4 weeks,followed by intranasal administration.Unsensitized control group immunized with 0.9%NaCl instead of ragweed pollen allergen. As a results, symptoms of allergic rhinitis,such as sneezing ,itching and rhinorrhea were observed in 28 guinea pigs of sensitized groups.There were significant increases in the number of eosinophils and Mast cell in the epithelium after 30 min in the sensitized group received intranasal ragweed pollen allergen. So, An animal model of allergic rhinitis induced by ragweed pollen allergen was established successfully.It provides a tool for studying and treating allergic rhinitis,especially induced by ragweed pollen allergen.
出处
《上海实验动物科学》
2001年第4期212-214,225,共4页
Shanghai Laboratory Animal Science
