摘要
目的 :研究原因不明性肝炎患者输血传播性病毒 (Transfusiontransmittedvirus,TTV)感染的检出状况及其临床意义。方法 :在TTVORF1 保守区设计引物 ,建立巢式聚合酶链反应 (Nested PCR) ,检测 5 0例原因不明性肝炎、40例健康人与 30例乙肝病毒携带者血清中TTVDNA ,比较TTVDNA阳性的肝炎患者的临床特征。结果 :5 0例原因不明性肝炎中 16例TTVDNA呈阳性 (占 32 % ) ,比健康人群 (2 40 ,5 % )与乙肝病毒携带者 (2 30 ,6 .7% )血清中TTVDNA检出率高 (P <0 .0 5 ) ,16例TTVDNA阳性患者中以慢性肝炎占多数 (12 16 ,75 .5 % ) ,临床表现为反复、持续ALT异常。结论 :TTV在原因不明性肝炎中感染率明显高于其他人群 ,其可能是本地区原因不明性肝炎的致病因子。
Objective:To investigate the presence of TTV infection among patients with hepatitis of unknown etiology and its clinical significance.Methods:A nested polymerase chain reaction(PCR) assay with primers from ORF1 of TTV genome was established to detect TTV DNA in 50 patients with hepatitis of unknown etiology,in 40 health persons and 30 HBV carriers.Results:The positive rate of TTV DNA accounted for 32%(16/50) in patients with hepatitis of unknown etiology,compared with the positive rate of TTV DNA accounting for 5%(2/40) in health persons and 6.7%(2/30) in HBV carriers. It had statistical difference(P<0.05). Among 16 patients with TTV DNA positive response,12 cases were chronic hepatitis(12/16,75%).Conclusion:TTV infection will be one of the major causes of hepatitis of unknown etiology in this area.
出处
《温州医学院学报》
CAS
2001年第6期360-361,364,共3页
Journal of Wenzhou Medical College
基金
温州市科委医药科学基金项目 (S2 0 0 0A17)
