摘要
目的 研究 ８Ｈｚ（１１０ｄＢ）次声作用对大鼠视网膜组织中ＧＦＡＰ和ＶＥＧＦ的蛋白表达变化情况。方法 把大鼠置于８Ｈｚ（１１０ ｄＢ）的次声压力舱中，每天暴露２ ｈ，于０、１、７、１４、２１天后，以免疫组化法检测视网膜组织中的ＧＦＡＰ、ＶＥＧＦ的蛋白表达。结果 次声作用７天后大鼠视网膜组织中的ＧＦＡＰ和ＶＥＧＦ的表达显著增强（Ｐ＜０．０５），１４、２１天后此２种蛋白的表达非常显著增强（Ｐ＜０．０１）。结论 ８Ｈｚ（１１０ ｄＢ）次声作用条件下可造成视网膜组织的一定影响，引起视网膜组织中胶质细胞、血管内皮细胞的增生，程度与作用时间呈正相关。
Objective To study the expression of GFAP and VEGF on rats retina by infrasounded injruy. Method Rats were exposed to 8Hz、110 dB SPL (2h/d) for 0, 1,7, 14 and 21 days.The expression of GFAP and VEGF in retina of rats were observed by using immunohistochmistry. Results After the rats were exposed to infrasound 7days, the expression of GFAP and VEGF increased compared to the control group (P < 0.05). And, after the rats were axposed for 14 and 21 days the two proteins increased singificantly (P < 0. 01). Conclusion The experimental condition of 8Hz, 110 dB SPL could induce the glial and vascular endothelial cells damage in rat retina. The injury are consistent with the prolongation of the exposed time.
出处
《眼外伤职业眼病杂志》
北大核心
2002年第1期6-8,共3页
Journal of Injuries and Occupational Diseases of the Eye with Ophthalmic Surgeries
基金
国家自然科学基金资助 编号:39900162
