摘要
研究了不同浓度的Hg2 +对萍蓬草离体类囊体膜的光合电子传递活性、吸收光谱、荧光发射光谱、多肽组分的影响及超微结构的毒害。结果表明 ,Hg2 +对 2个光系统的电子传递活性均有抑制作用 ,且对PSⅠ的抑制作用较PSⅡ大。Hg2 +处理导致类囊体膜在红光和蓝紫光区域的吸收峰都有下降 ,类囊体膜在波长 685nm处的荧光发射峰随Hg2 +浓度升高也呈下降趋势。Hg2 +处理并未使类囊体膜的多肽组分发生明显变化。在 1 0mmol·L- 1Hg2 +毒害下 ,类囊体片层结构肿涨 ,基质减少 ,且随着毒害时间的延长 ,类囊体片层结构肿涨加剧 ,进而解体 ,基质进一步减少。
Effects of Hg 2+ ,varied with concentration,on electron transport activity of photosystem,absorption spectrum,fluorescence emission spectrum at room temperature,polypeptide compositions and ultrastructure of thylakoid membrane of Nuphar pumilum DC.were studied. Hg 2+ inhibited the electron transport activity of PSⅠ and PSⅡ and the effect was stronger on PSⅠ than on PSⅡ.For the Hg 2+ treatment,its absorption peaks were at 430 nm and 680 nm and its fluorescence peak,too,at 685 nm,but the Hg 2+ treatment did not affect its polypeptide composition.Treatment with 10 mmol·L -1 Hg 2+ caused thylakoid swell and stroma reduce in chloroplast.With the time of the treatment extending,thylakoid swelled further and eventually decomposed. As a consequence, stroma further reduced as well.
出处
《农村生态环境》
CAS
CSSCI
CSCD
北大核心
2002年第1期26-30,共5页
Rural Eco-Environment
基金
国家自然科学基金(39770046)
江苏省教育委员会高校科研项目(1999SWX0000SJ1)
关键词
Hg^(2+)
萍蓬草
类囊体膜
电子传递活性
多肽组分
超微结构
Hg^(2+)Nuphar pumilumDC.
thylakoid membrane
?electron transport activity
polypeptide compositions
ultrastructure
