摘要
【目的】探讨幽门螺杆菌及其空泡毒素基因 (vacA)亚型、细胞毒素相关基因 (cagA)与胃上皮HLA DR抗原表达间的关系。【方法】①自 39例幽门螺杆菌阳性患者中分离培养出 39株幽门螺杆菌菌株 ,采用PCR方法鉴定 39株菌株的ca gA及vacA亚型 ;②采用HLA DR小鼠抗人单克隆抗体 ,对上述已行cagA及vacA亚型鉴定的幽门螺杆菌阳性患者及 2 2例阴性患者的胃窦活检标本行免疫组化染色。【结果】①幽门螺杆菌阳性患者胃上皮HLA DR表达较阴性患者更显著 ;②幽门螺杆菌定植密度与胃上皮HLA DR抗原表达程度间存在正相关 ;③感染cagA+ 菌株患者较感染cagA-株者胃上皮HLA DR抗原表达更明显 ;④本研究中vacAs1a/m2亚型占 90 % ,故未对不同vacA亚型与胃上皮HLA DR表达间的关系进行统计分析。【结论】①幽门螺杆菌感染可诱导胃上皮HLA DR抗原异常表达 ;②cagA+ 菌株可能通过胃上皮HLA Ⅱ类分子介导而与宿主发生更为密切的相互作用 ,从而较cagA-菌株引起更为显著的胃上皮损伤。
To investigate the relationship between Helicobacter pylori(H.pylori), its vacA subtypes, cagA and HLA DR antigen expression in gastric epithelia. ①Isolating 39 H.pylori strains from 39 H.pylori positive patients, and identifying the cagA and vacA subtypes using PCR method. ②39 and 22 pathologic samples biopsied from H.pylori positive patients whose H.pylori gene types had already been identified and H.pylori negative patients respectively were sectioned, then processed by immuno histo chemistry stain using monoclonal antibody aim for HLA DR antigen. Analyze the link between H.pylori infection, its vacA subtypes, cagA and the degree of HLA DR antigen expression in gastric antral epithelia. ①There was significantly more extensive HLA DR antigen expression in gastric epithelia in H.pylori positive patients than that existed in H.pylori negative patients.② There was positive correlation between the degree of HLA DR antigen expression in gastric epithelia and the degree of bacterial density.③ There was significantly more extensive HLA DR antigen expression in gastric epithelia in cagA + patients than that existed in cagA - patients.④ In this study, vacA s1a/m2 subtype is predominant(90%), then the relationship between vacA subtypes and HLA DR antigen expression in gastric epithelia was not analyzed. [Conclusions] Mediated by HLA Ⅱ molecules expressed in gastric epithelia, cagA + strains might have closer interaction with hosts, and induce more severe injury of gastric epithelia than cagA - strains.
出处
《中山医科大学学报》
CSCD
北大核心
2002年第1期56-59,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
广东省科委自然科学基金资助项目 ( 970 46 )
广东省医学科研基金资助项目 ( 2 0 0 0 0 2 6 )
