摘要
目的 为了探讨高糖对血管紧张素Ⅱ促系膜细胞增殖作用的影响及其机制。方法 采用体外人肾小球系膜细胞培养 ,系膜细胞增殖能力采用MTT法 ,蛋白激酶C(PKC)α、β2 、ε表达应用免疫细胞化学检测。结果 高糖对AngⅡ促系膜细胞增殖有正相调节作用 ,高糖和AngⅡ可刺激系膜细胞PKCβ2 表达增强 ,PKC抑制剂可阻断高糖和AngⅡ的促增殖作用。结论 高糖和AngⅡ促系膜细胞增殖作用是通过PKC依赖途径介导的 ,应用PKC抑制剂可能是防治糖尿病肾病发生发展的手段之一。
Objective To study the effect of high glucose and its mechanism on proliferation of mesangial cell by angiotensinⅡ(AngⅡ) Methods The culture of human mesangial cell(MC)was established,MTT method was used to measure of human mesagial cell(MC),the expression of protein kinase C was detected by immunocytochemistry Results The proliferation of MC by AngⅡwas regulated positively by high glucose,high glucose and AngⅡcan significantly stimulate the expression of PKCβ2 in MC,the inhibitor of PKC can break the proliferation of MC by high glucose and AngⅡ Conclusion The proliferation of MC by high glucose and AngⅡwas mediated by PKC dependent pathway,the inhibitor of PKC may be one of means prevented diabetic nephropathy
出处
《重庆医学》
CAS
CSCD
2002年第2期89-90,共2页
Chongqing medicine
关键词
葡萄糖
血管紧张素Ⅱ
系膜细胞
增殖
机制
glucose
angiotensin Ⅱ
mesangial cell
proliferation
mechanism
